PISSARO

URL of the website: http://plateforme-proteomique.crihan.fr

Chief Scientific Officer: Pascal Cosette Chief Technical Officer: David Vaudry Quality managers: Laurent Coquet, Clémence Châtelain

Contact

PISSARO
Centre universitaire de recherche et d’innovation en biologie (CURIB)
25 rue Tesnière
76821 Mont-Saint-Aignan
Phone: +33 235 14 6397
pascal.cosette@univ-rouen.fr

Created in 2003, the PISSARO platform offers the scientific, academic and industrial community, expertise and effective and recognised technological resources in the field of separation, identification and quantification of proteins and peptides, as well as in the study of their post-translational modifications.

The platform is equipped with a fleet of instruments combining many techniques: mass spectrometry, liquid chromatography, N-terminal sequencing, one- and two-dimensional electrophoresis, etc. A dedicated staff ensures control of the equipment, as well as the preparation of samples in all areas of the living world. This know-how is used to carry out or contribute to research projects on various organisms: bacteria, humans and plants.

Means and equipments

Mass spectrometry and chromatography:

• EASY-nLC and LTQ-Orbitrap Elite coupling (Thermo Scientific),
• Ultimate 3000 and Q-Exactive coupling (Thermo Scientific),
• Nano-LC, Chip and QTOF XT coupling (Agilent),
• Capillary electrophoresis and QTOF 6520 coupling (Agilent),
• UPLC 1290 and QQQ 6490 coupling with iFunnel technology (Agilent),
• MALDI-TOF/TOF UltrafleXtreme mass spectrometer (Bruker).

• Procise 494 sequencer (Applied Biosystems),
• Procise 492 sequencer (Applied Biosystems).

• Systems for isoelectric focusing: IPGPhor (GE Healthcare), Multiphore II (Pharmacia), IEFCell (BioRad) and offGel (Agilent),
• Systems for SDS-PAGE: PROTEAN II XL (BioRad) and Dodeca Cell Criterion (BioRad),
• Typhoon FLA 9500 biomolecular imager (GE Healthcare).

• Multiprobe II automaton (Perkin Elmer),
• Biomek3000 PLC (Beckman),
• JANUS automaton (Perkin Elmer).

• Protein identification: Mascot (Matrix Sciences), Proteome Discoverer (Thermo Scientific) and Spectrum Mill (Agilent),
• De novo sequencing: Peaks Studio (Bioinformatics Solutions),
• Quantification: Progenesis QI (Waters), SameSpots (TotalLab) and Mass Profiler (Agilent),
• Functional annotation: IPA (Qiagen).

• Translational research and identification of biomarkers for diagnostic, prognostic, theranostic purposes, in particular in the context of autoimmune diseases,
• Characterization of glycoproteins, in particular glycosylated therapeutic proteins,
• Search for protein targets involved in adhesion and bacterial resistance,
• Expertise development in the field of post-translational modifications,
• Research and quantification

• Lopez AG, Duparc C, Wils J, Naccache A, Castanet M, Lefebvre H and Louiset E. Steroidogenic cell microenvironment and adrenal function in physiological and pathophysiological conditions. Mol Cell Endocrinol 2021, 535: 111377.
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• Nicola C, Dubois M, Campart C, Al Sagheer T, Desrues L, Schapman D, Galas L, Lange M, Joly F and Castel H. The Prostate Cancer Therapy Enzalutamide Compared with Abiraterone Acetate/Prednisone Impacts Motivation for Exploration, Spatial Learning and Alters Dopaminergic Transmission in Aged Castrated Mice. Cancers (Basel) 2021, 13: 3518.
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• Corbière A, Walet-Balieu ML, Chan P, Basille-Dugay M, Hardouin J and Vaudry D. A Peptidomic Approach to Characterize Peptides Involved in Cerebellar Cortex Development Leads to the Identification of the Neurotrophic Effects of Nociceptin. Mol Cell Proteomics 2018, 17: 1737-1749.
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• Nobis S, Goichon A, Achamrah N, Guérin C, Azhar S, Chan P, Morin A, Bôle-Feysot C, do Rego JC, Vaudry D, Déchelotte P, , Belmonte L, and Coëffier M,. Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia. Sci Rep 2018, 8: 7233.
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• Péden R, Rocher B, Chan P, Vaudry D, Poret A, Olivier S, Le Foll F and Bultelle F. Highly polluted life history and acute heat stress, a hazardous mix for blue mussels. Mar Pollut Bull 2018, 135: 594-606.
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• Cases O, Obry A, Ben-Yacoub S, Augustin S, Joseph A, Toutirais G, Simonutti M, Christ A, Cosette P and Kozyraki R. Impaired vitreous composition and retinal pigment epithelium function in the FoxG1::LRP2 myopic mice. Biochim Biophys Acta Mol Basis Dis 2017, 1863: 1242-1254.
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• Porte B, Hardouin J, Zerdoumi Y, Derambure C, Hauchecorne M, Dupre N, Obry A, Lequerre T, Bekri S, Gonzalez B, Flaman JM, Marret S, Cosette P and Leroux P. Major remodeling of brain microvessels during neonatal period in the mouse: A proteomic and transcriptomic study. J Cereb Blood Flow Metab 2017, 37: 495-513.
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• Arfi Y, Minder L, Di Primo C, Le Roy A, Ebel C, Coquet L, Claverol S, Vashee S, Jores J, Blanchard A and Sirand-Pugnet P. MIB-MIP is a mycoplasma system that captures and cleaves immunoglobulin G. Proc Natl Acad Sci U S A 2016, 113: 5406-11.
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• Breton J, Tennoune N, Lucas N, Francois M, Legrand R, Jacquemot J, Goichon A, Guérin C, Peltier J, Pestel-Caron M, Chan P, Vaudry D, do Rego JC, Liénard F, Pénicaud L, Fioramonti X, Ebenezer IS, Höfelt T, Déchelotte P and Fetissov SO. Gut Commensal E. coli Proteins Activate Host Satiety Pathways following Nutrient-Induced Bacterial Growth. Cell Metab 2016, 23: 324-34.
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