PRIMACEN

URL of the website: http://primacen.fr

Chief Scientific Officer: David Vaudry Chief Technical Officer: Ludovic Galas Quality managers: Ludovic Galas, Magalie Bénard

Contact

PRIMACEN Université de Rouen Normandie, UFR Sciences et Techniques,
Centre Universitaire de Recherche et d’Innovation en Biologie (CURIB)

25, rue Lucien Tesnière
76821 Mont-Saint-Aignan cedex

Phone: 02-35-14-70-48

contact@primacen.fr

The PRIMACEN platform brings together four services: peptide synthesis and functional screening, photonic bio-imaging, transmission electron microscopy, microdissection and Q-PCR. It thus offers a continuum, from the synthesis of biomarkers and the preparation of samples to the localisation and determination of the biological activity of molecules of interest.
PRIMACEN has equipment for the synthesis, purification and characterisation by mass spectrometry of peptides and peptide analogues. The platform also offers the necessary equipment for the preparation of cells (culture) and tissues (sections, slices, microdissection) of various origins (bacterial, plant, animal or human), fixed or alive, and intended in particular for imaging. A wide spectrum of multidimensional resolutions is accessible thanks to light microscopy, light macroscopy and transmission electron microscopy. PRIMACEN also offers the possibility of determining the biological activity of molecules of interest via medium-throughput screening methods (bioassays) and the quantification of relative gene expression thanks to transcriptomic analyses (Q-PCR).
PRIMACEN is also involved in the development of computerised applications in support of academic structures and in many fields: communication, management of publications, deliverables, real estate assets, inventories, congresses, etc.

Means and equipments

Peptide synthesis and functional screening:

• 3 peptide synthesizers,
• 4 HPLCs,
• 2 MALDI-TOF mass spectrometers,
• 2 plate readers: Flexstation 3 and Victor V.

• STED-FLIM inverted confocal microscope with white laser (Leica),
• Upright confocal microscope with fixed stage SP8 MP (Leica) with Chameleon infrared pulsed laser (Coherent),
• Inverted confocal microscope gCW STED SP5 X with white laser (Leica),
• Confocal macroscope and wide field LSI (Leica),
• Videomicroscopes: TIRF (Leica), BioStation (Nikon), Cell Discoverer 7 (Zeiss),
• Wide field microscopy systems, bright and dark background, epifluorescence (Nikon),
• Axioscope 7 microscopy system (Zeiss),
• AxioZoom wide field, brightfield and fluorescence (structured illumination) macroscope (Zeiss),
• Brightfield and darkfield macroscope (Leica),
• Complete Imaris image analysis systems (Bitplane) and Huygens Professional (SVI) deconvolution systems,
• Laboratory L2 cell culture (animal cells).

• HPM100 high pressure freezing system (Leica),
• Automated AFS2 freeze substitution machine (Leica),
• UC6 Ultramicrotome and UC7 cryo-ultramicrotome (Leica),
• Tecnai 12 (FEI) transmission electron microscope,
•   Laboratory L2 cell culture (plant cells).

• 3 microtome cryostats CM3050 and CM1950 (Leica), NX70 (Microm, Thermo Scientific),
• 2 vibrating blade microtomes VT1000 (Leica),
• 2 microdissection microscopes (Leica, PALM Zeiss),
• 3 quantitative PCR (Q-PCR) and digital PCR devices,
• 1 Bravo robot (Agilent).

• Development of pharmacological, therapeutic and diagnostic tools,
• Study of cell signalling, proliferation and apoptosis by bioassays,
• High and super spatial and temporal resolution fluorescence imaging,
• Image processing and analysis: deconvolution and multidimensional modelling,
• Transmission electron and cryogenic electron microscopy,
• Laser microdissection coupled with high-throughput real-time PCR expression analysis,
• Development of computer applications.

• El Mehdi M, Takhlidjt S, Devère M, Arabo A, Le Solliec MA, Maucotel J, Bénani A, Nedelec E, Duparc C, Lefranc B, Leprince J, Anouar Y, Prévost G, Chartrel N and Picot M. The 26RFa (QRFP)/GPR103 neuropeptidergic system in mice relays insulin signalling into the brain to regulate glucose homeostasis. Diabetologia 2022, 65(7): 1198-1211.
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• Faklaris O, Bancel-Vallée L, Dauphin A, Monterroso B, Frère P, Geny D, Manoliu T, de Rossi S, Cordelières FP, Schapman D, Nitschke R, Cau J and Guilbert T. Quality assessment in light microscopy for routine use through simple tools and robust metrics. J Cell Biol 2022, 221(11): e202107093.
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• Heron C, Dumesnil A, Houssari M, Renet S, Lemarcis T, Lebon A, Godefroy D, Schapman D, Henri O, Riou G, Nicol L, Henry JP, Valet M, Pieronne-Deperrois M, Ouvrard-Pascaud A, Hägerling R, Chiavelli H, Michel JB, Mulder P, Fraineau S, Richard V, Tardif V and Brakenhielm E. Regulation and impact of cardiac lymphangiogenesis in pressure-overload-induced heart failure. Cardiovasc Res 2022, 086
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• Leroux S, Rodriguez-Duboc A, Arabo A, Basille-Dugay M, Vaudry D and Burel D. Intermittent hypoxia in a mouse model of apnea of prematurity leads to a retardation of cerebellar development and long-term functional deficits. Cell Biosci 2022, 12(1): 148.
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• Ropitaux M, Hays Q, Baron A, Fourmois L, Boulogne I, Vauzeilles B, Lerouge P, Mollet JC and Lehner A. Dynamic imaging of cell wall polysaccharides by metabolic click-mediated labeling of pectins in living elongating cells. Plant J 2022, 110(3): 916-924.
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• Bénard M, Schapman D, Chamot C, Dubois F, Levallet G, Komuro H and Galas L. Optimization of Advanced Live-Cell Imaging through Red/Near-Infrared Dye Labeling and Fluorescence Lifetime-Based Strategies. Int J Mol Sci 2021, 22: 11092.
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• Galas L, Burel C, Schapman D, Ropitaux M, Bernard S, Bénard M and Bardor M. Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain. Front Plant Sci 2021, 12: 638181.
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• Komuro Y, Galas L, Morozov YM, Fahrion JK, Raoult E, Lebon A, Tilot AK, Kikuchi S, Ohno N, Vaudry D, Rakic P and Komuro H. The Role of Galanin in Cerebellar Granule Cell Migration in the Early Postnatal Mouse during Normal Development and after Injury. J Neurosci 2021, 41: 8725-8741.
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• Le H, Arnoult C, Dé E, Schapman D, Galas L, Le Cerf D and Karakasyan C. Antibody-Conjugated Nanocarriers for Targeted Antibiotic Delivery: Application in the Treatment of Bacterial Biofilms. Biomacromolecules 2021, 22: 1639-1653.
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• Lopez AG, Duparc C, Wils J, Naccache A, Castanet M, Lefebvre H and Louiset E. Steroidogenic cell microenvironment and adrenal function in physiological and pathophysiological conditions. Mol Cell Endocrinol 2021, 535: 111377.
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• Carmon O, Laguerre F, Riachy L, Delestre-Delacour C, Wang Q, Tanguy E, Jeandel L, Cartier D, Thahouly T, Haeberlé AM, Fouillen L, Rezazgui O, Schapman D, Haefelé A, Goumon Y, Galas L, Renard PY, Alexandre S, Vitale N, Anouar Y and Montero-Hadjadje M. Chromogranin A preferential interaction with Golgi phosphatidic acid induces membrane deformation and contributes to secretory granule biogenesis. FASEB J 2020, 34: 6769-6790.
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• Chalfouh C, Guillou C, Hardouin J, Delarue Q, Li X, Duclos C, Schapman D, Marie JP, Cosette P and Guérout N. The Regenerative Effect of Trans-spinal Magnetic Stimulation After Spinal Cord Injury: Mechanisms and Pathways Underlying the Effect. Neurotherapeutics 2020
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• Delarue Q, Mayeur A, Chalfouh C, Honoré A, Duclos C, Giovanni MD, Li X, Salaun M, Dampierre J, Vaudry D, Marie JP and Guérout N. Inhibition of ADAMTS-4 Expression in Olfactory Ensheathing Cells Enhances Recovery after Transplantation within Spinal Cord Injury. J Neurotrauma 2020
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• Houssari M, Dumesnil A, Tardif V, Kivelä R, Pizzinat N, Boukhalfa I, Godefroy D, Schapman D, Hemanthakumar KA, Bizou M, Henry JP, Renet S, Riou G, Rondeaux J, Anouar Y, Adriouch S, Fraineau S, Alitalo K, Richard V, Mulder P and Brakenhielm E. Lymphatic and Immune Cell Cross-Talk Regulates Cardiac Recovery After Experimental Myocardial Infarction. Arterioscler Thromb Vasc Biol 2020
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• Wils J, Duparc C, Cailleux AF, Lopez AG, Guiheneuf C, Boutelet I, Boyer HG, Dubessy C, Cherifi S, Cauliez B, Gobet F, Defortescu G, Mé, nard JF, Louiset E, Lefebvre H and . The neuropeptide substance P regulates aldosterone secretion in human adrenals. Nat Commun 2020, 11: 2673.
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• Arkoun B, Galas L, Dumont L, Rives A, Saulnier J, Delessard M, Rondanino C and Rives N. Vitamin E but Not GSH Decreases Reactive Oxygen Species Accumulation and Enhances Sperm Production during In Vitro Maturation of Frozen-Thawed Prepubertal Mouse Testicular Tissue. Int J Mol Sci 2019, 20:  E5380.
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• Léger C, Dupré N, Aligny C, Bénard M, Lebon A, Henry V, Hauchecorne M, Galas L, Frebourg T, Leroux P, Vivien D, Lecointre M, Marret S and Gonzalez BJ. Glutamate controls vessel-associated migration of GABA interneurons from the pial migratory route via NMDA receptors and endothelial protease activation. Cell Mol Life Sci 2019
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• Alim K, Lefranc B, Sopkova-de Oliveira Santos J, Dubessy C, Picot M, Boutin JA, Vaudry H, Chartrel N, Vaudry D, Chuquet J and Leprince J. Design, Synthesis, Molecular Dynamics Simulation, and Functional Evaluation of a Novel Series of 26RFa Peptide Analogues Containing a Mono- or Polyalkyl Guanidino Arginine Derivative. J Med Chem 2018, 61: 10185-10197.
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• El Amki M, Dubois M, Lefevre-Scelles A, Magne N, Roussel M, Clavier T, Guichet PO, Gérardin E, Compère V, and Castel H,. Long-Lasting Cerebral Vasospasm, Microthrombosis, Apoptosis and Paravascular Alterations Associated with Neurological Deficits in a Mouse Model of Subarachnoid Hemorrhage. Mol Neurobiol 2018, 55: 2763-2779.
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• Benjdia A, Guillot A, Ruffié P, Leprince J and Berteau O. Post-translational modification of ribosomally synthesized peptides by a radical SAM epimerase in Bacillus subtilis. Nature Chem 2017, 9: 698-707.
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• Schapman D, Perraudeau C, Bénard M, Gallavardin T, Boulangé A, Leleu S, Lebon A, Franck X, Galas L. Characterization of fluorescent synthetic epicocconone-based dyethrough advanced light microscopies for live cell imaging applications. DYES PIGMENTS 2017, 141: 394-405.